DGHM
EINE STARKE GEMEINSCHAFT

Molecular surveillance of multidrug-resistant Gram-negative bacteria in Ukrainian patients, Germany, March to June 2022

Autor:innen:
Tilman Schultze^1,2,3,* , Michael Hogardt^1,2,3,* , Erwin Sanabria Velázquez^1,2,3 , Daniel Hack^1,2,3 , Silke Besier^1,2,3 , Thomas A Wichelhaus^1,2,3 , Ulrich Rochwalsky⁴ , Volkhard AJ Kempf^1,2,3,** , Claudia Reinheimer^1,2,3,**

Tilman Schultze                         Claudia Reinheimer

Abstract:
Background:
Since the beginning of the war in Ukraine in February 2022, Ukrainians have been seeking shelter in other European countries.
Aim:
We aimed to investigate the prevalence and the molecular epidemiology of multidrug-resistant Gram-negative (MDRGN) bacteria and meticillin-resistant Staphylococcus aureus (MRSA) in Ukrainian patients at admittance to the University Hospital Frankfurt, Germany.
Methods: We performed screening and observational analysis of all patients from March until June 2022. Genomes of MDRGN isolates were analysed for antimicrobial resistance, virulence genes and phylogenetic relatedness.
Results:
We included 103 patients (median age: 39 ± 23.7 years), 57 of whom were female (55.3%; 95% confidence interval (CI): 45.2–5.1). Patients were most frequently admitted to the Department of Paediatrics (29/103; 28.2%; 95% CI: 19.7–37.9). We found 34 MDRGN isolates in 17 of 103 patients (16.5%; 95% CI: 9.9–25.1). Ten patients carried 21 carbapenem-resistant (CR) bacteria, five of them more than one CR isolate. Four of six patients with war-related injuries carried eight CR isolates. In six of 10 patients, CR isolates caused infections. Genomic characterisation revealed that the CR isolates harboured at least one carbapenemase gene, blaNDM-1 being the most frequent (n = 10). Core genome and plasmid analysis revealed no epidemiological connection between most of these isolates. Hypervirulence marker genes were found in five of six Klebsiella pneumoniae CR isolates. No MRSA was found.
Conclusion:
Hospitals should consider infection control strategies to cover the elevated prevalence of MDRGN bacteria in Ukrainian patients with war-related injuries and/or hospital pre-treatment and to prevent the spread of hypervirulent CR isolates.

Kommentar:
Dies ist die erste molekulargenetisch-epidemiologische Analyse zu multiresistenten gramnegativen Bakterien bei ukrainischen Patienten an einem deutschen Universitätsklinikum. Bei ukrainischen Patienten findet sich eine hohe Prävalenz multiresistenter gramnegativen Bakterien, in erheblichem Anteil mit Carbapenem-Resistenz, sowie das Auftreten hypervirulenter Isolate. Es bedarf einer situations-adaptierten krankenhaushygienischen Strategie, um die Ausbreitung der Pathogene in Kliniken und die hiermit einhergehende Patientengefährdung zu vermeiden.

Kontakt:
Claudia Reinheimer (claudia.reinheimer@kgu.de)

Autor:inneninformationen:
¹ Institute for Medical Microbiology and Infection Control, University Hospital Frankfurt, Frankfurt am Main, Germany
² University Centre of Infectious Diseases, University Hospital Frankfurt, Frankfurt am Main, Germany
³ University Centre of Competence for Infection Control of the State of Hesse, Frankfurt am Main, Frankfurt Main, Germany
⁴ Department of Paediatrics, University Hospital Frankfurt, Frankfurt am Main, Germany

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